This animated film highlights each voice of the fugue visually.
It has also been used in many film scores, both as diegetic and non-diegetic music.
Conductor Leopold Stokowski arranged it for the opening scene of Disney’s original Fantasia (1940):
Here it is in a scene from Sunset Boulevard (1950):
Here it is in 20,000 Leagues Under the Sea (1954):
In The Great Race (1965):
In Monty Python’s The Meaning of Life (1983):
In The Aviator (2004), in Stokowski’s orchestral transcription: